The ADAM-MC, an automated cell counter, performs viability and cell counting measurements using Propidium Iodide (PI) staining method of dead-cell staining combined with advanced image analysis produces highly repeatable results similar to a flow cytometer, but at a fraction of the cost.
Until now, cell counting and viability measurement for many types of cells have been performed manually using a hemocytometer with Trypan Blue exclusion method, which is to distinguish viable cells from non-viable cells. One drawback of this method, however, is the propensity for the staining of artifacts; another drawback is that the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber. This combined with the potential problem of cell aggregation and limited sample volume, leads to the common variation of counts normally associated with this method. To address these problems, NanoEnTek has developed the ADAM-MC, which is based on a fluorescent microscopy technique for counting cells. The ADAM-MC utilizes sensitive fluorescence dye staining, LED, optics and CCD detection technologies to make the cell analysis more accurate and reliable. Counting cells is easily performed using ADAM-MC by mixing cells with a Propidium Iodide (PI) stain and directly pipetting them on to a disposable plastic chip. The chip is then loaded onto a precision stage. ADAM-MC system is automatically focuses onto the chip and cells that have been stained are recorded by a sensitive CCD camera. The image results are automatically processed generating the cell count which is displayed on the front of the instrument. Simple – Fast – Accurate – Reliable.
ADAM-MC is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. ADAM-MC provides two kinds of staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the ADAM-MC software after each measurement of the total cells and the nonviable cells.