ADAM-MC – New Standard of Automated Cell Counter

//ADAM-MC – New Standard of Automated Cell Counter

ADAM-MC – New Standard of Automated Cell Counter

$8,195.00

The ADAM-MC, an automated cell counter, performs viability and cell counting measurements using Propidium Iodide (PI) staining method of dead-cell staining combined with advanced image analysis produces highly repeatable results similar to a flow cytometer, but at a fraction of the cost.

Until now, cell counting and viability measurement for many types of cells have been performed manually using a hemocytometer with Trypan Blue exclusion method, which is to distinguish viable cells from non-viable cells. One drawback of this method, however, is the propensity for the staining of artifacts; another drawback is that the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber. This combined with the potential problem of cell aggregation and limited sample volume, leads to the common variation of counts normally associated with this method. To address these problems, NanoEnTek has developed the ADAM-MC, which is based on a fluorescent microscopy technique for counting cells. The ADAM-MC utilizes sensitive fluorescence dye staining, LED, optics and CCD detection technologies to make the cell analysis more accurate and reliable. Counting cells is easily performed using ADAM-MC by mixing cells with a Propidium Iodide (PI) stain and directly pipetting them on to a disposable plastic chip. The chip is then loaded onto a precision stage. ADAM-MC system is automatically focuses onto the chip and cells that have been stained are recorded by a sensitive CCD camera. The image results are automatically processed generating the cell count which is displayed on the front of the instrument. Simple – Fast – Accurate – Reliable.

ADAM-MC is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. ADAM-MC provides two kinds of staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the ADAM-MC software after each measurement of the total cells and the nonviable cells.

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Description

New Standard of Automatic Cell Counting ADAM-MC – NanoEnTek ADAM series automatic cell counter measures total cell numbers and cell viabilities through cutting-edge detection technologies. Instead of tryphan blue staining which can lead to inaccurate data, ADAM utilizes sensitive fluorescence dye staining, LED optics, and CCD detection technologies to make cell analysis more accurate and reliable.

Combined with a disposable microchip, operation of the ADAM-MC automatic cell counter is now extremely simple, easy to use, and cost effective. The need for routine calibration of the devise is completely eliminated. Cleaning and warm-up time is no longer necessary with ADAM. The disposable chip greatly reduces the risk of exposure to hazardous samples, such as patient’s blood samples, virus infected cells, etc.

New Technology behind the Accuracy of ADAM – ADAM automatic cell counter is a precision microscope integrated with fluorescence optics and image analysis software. After the samples are stained with fluorescent dye, Propidium Iodide (PI), which intercalates DNA to stain the nucleus of the target cells, fluorescent images are taken automatically. The system takes 20 – 60 images by movement of an automatic X–Y stage. The obtained images are processed by image analysis software integrated inside of the system. All procedures are automated once the stained sample has been pipetted onto the disposable chip.

  • LED excitation
  • Precision automatic stage
  • Automatic focusing
  • Sensitive CCD detection
  • Automatic image analysis
  • Large counting volume guarantees accurate results

Disposable Microchips – Conventional automatic cell counters utilize a flow cell or counting chamber, which is permanently integrated inside the devise. During or after cell counting, tubing or the counting chamber can become clogged by cell debris. Therefore, conventional automatic cell counters require necessary routine maintenance and cleaning.

ADAM automatic cell counter utilizes a precision disposable microchip which eliminates problems associated with permanent counting chambers and tubing. Each microchip is designed for single use. Pumping, cleansing, and waste collection is not required as with conventional automatic cell counters. Also, the risk of exposure to potential hazardous materials, such as patients’ blood samples, is completely eliminated with disposable chips. Each microchip is produced in a state-of-the-art manufacturing facility, and is validated for accuracy.

Design You Must Have – ADAM automatic cell counter has been designed with the user in mind. ADAM has a compact bench-top ergonomic design that is easy to use. Operation is simple, and can be quickly learned by any technician, regardless of skill level.

  • Ergonomic design
  • Small and compact
  • Plug and play technology
  • Easy operation
  • External monitor to view cell images
  • No maintenance required

Data You Can Trust – Accuracy & repeatability.

As accurate as FACS counting: Cell counting accuracy depends on the counting volume. 8.5µL counting is 8.5 times more accurate than counting 1µL analysis. ADAM analyzes up to 60 images which is equivalent to 8.5µL counting volume.

These improvements specifically address variation attributable to low sample reads (some competitor models only perform one read), low sample volumes (most models only read 1µL or less), and inaccurate resolution of cells (often found when cells clump together). The results are amazing when using the ADAM automatic cell counter and it is common to have R2 values over 0.99 and CVs < 1%.

Highly qualified technical support 24/7 by NanoEnTek technical application specialists (HQ) and Chinchilla Life technicians.

  • Qualified specialists are ready to support you. From free training on basic operation to experimental support, we can help.

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Specifications

Items Specifications
Power AC 100 – 240V, 50-60Hz
Size 220 (W) x 375 (W) x 250 (H) mm
Weight 9.0 Kg
Objective lens 4X
Light source Green LED 532 nm
Camera B/W CCD
Filter Excitation, Emission, Dichroic filter
Measuring range 5 x 104 – 4 x 106 cell/ml
Analysis time 45 sec/test (AccuChip 2X)
2 min/test (AccuChip 4X)
Loading sample volume 20µL/test (AccuChip 2X)
12µL/test (AccuChip 4X)
Measuring sample volume 8.5µL/test (AccuChip 2X)
3µL/test (AccuChip 4X)
Staining solution PI (Propidium Iodide)

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