Flame COVID-19 Variants Amplification Kit test 100

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qPCR Master Kit – CE-IVD

The kit is able to simultaneously detect the presence of COVID infection of both the “common” strain and the English, South African and Brazilian P2 and other Variant Of Concern with RT-PCR the method in a single “well”. Not Sequencing required!

This kit is designed with specific primers and Taqman probes for the New Coronavirus ORF1ab and the conservative region of the N gene sequence encoding the nucleocapsid protein of the new coronavirus to diagnose or differentially diagnose whether the tested patient is infected with the new coronavirus, the strain-specific N501Y mutation site and the strain-specific E484K mutation sites.

It’s available now the Flame DELTA VARIANT Reflex Reagent Kit for specific detection of all DELTA Variats. The Flame DELTA VARIANT Reflex Reagent Kit contains specific probes and positive controls and it’s just to be added to Flame CoViD-19 Variants qPCR Master kit in second level test in case of sample SARS-CoV-2 positive but N501Y-E484K negative.

References

1. SARS-CoV-2-variant-multiple-spike-protein-mutations-United-Kingdom, EuropeanCentreforDiseasePreventionandControl,Stockholm,2020.

https://www.ecdc.europa.eu/sites/default/files/documents/SARS-CoV-2-variant-multiple-spike-protein-mutations-United-Kingdom.pdf.

2. ScienceBrief:EmergingSARS-CoV-2Variants,CDC,Jan.28,2021.

https://www.cdc.gov/coronavirus/2019-ncov/more/science-and-research/scientific-brief-emerging-variants.html

3. WHO_SARS-CoV-2Variants,DiseaseOutbreakNews,WHO,31December2020.

https://www.who.int/csr/don/31-december-2020-sars-cov2-variants/en/

4. EarlytransmissibilityassessmentoftheN501YmutantstrainsofSARS-CoV-2intheUnitedKingdom,LeungKetal.OctobertoNovember2020.

EuroSurveill.2021Jan;26(1):2002106.doi:10.2807/1560-7917.ES.2020.26.1.2002106.PMID:33413740;PMCID:PMC7791602.

https://pubmed.ncbi.nlm.nih.gov/33413740/

5. ReducedneutralizationofSARS-CoV-2B.1.1.7variantbyconvalescentandvaccinesera,

PiyadaSupasaetal,Cell,2021,ISSN0092-8674,https://doi.org/10.1016/j.cell.2021.02.033

https://www.sciencedirect.com/science/article/pii/S0092867421002221.

Specifications

Box	48-100 Tests
Storage	-20°±5°, protected from light
Number of freezing and thawing allowed	not exceed 7 times
LoD	500 copies/ml
Positive agreement	100%
Negative agreement	100%

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