Arthur™

ASSAY #2

GFP expression assay

  Figure2. Comparison of GFP expression profile in two platforms

U-2 OS cells were transfected using the Neon transfection system (from Life Technologies) and 0.25 μg of the five different GFP constructs
and then analyzed on Arthur™ and competitor’s product F to determine transfection efficiency.
The histograms in panels A (Arthur™) and C (competitor’s product F) show the green fluorescence intensities and counts for the transfected populations.

The percentages of GFP-expressing cells detected with Arthur™ were consistent with the results from the competitor’s product F.

‘Colored circles’ option makes it easy to identify gated cells (Panel B); colored circles represent
: cell expressing GFP (green circles), non-GFP-expressing cells (blue circles).
The image in panel D shows the GFP expressing cells before trypsinization observed with a conventional fluorescence microscope.

ASSAY #3

Annexin V apoptosis

Figure3. Arthur™ allows confirmation of apoptosis assay using the visual display of the samples.

Following 18 hour-treatment of Staurosporine, HeLa cells were stained with apoptosis kit and subjected to Arthur™ and competitor’s product F.

Apoptosis Kit contains an annexin V-Alexa Fluor 488 conjugate and PI to differentiate
Live (Annexin V-negative / PI-negative), Dead (PI-positive) and Apoptotic (Annexin V-positive / PI-negative) cells.

ASSAY #4

Cell cycle assay

Figure4. Comparison of cell cycle profiles in two platforms : Arthur™ and competitor’s Product F

HeLa cells were treated with Nocodazole (Microtubule-Destabilizer) or Epothilone B (Microtubule-Stabilizer) to arrest cells in G2/M phase.
Cells were fixed with 70% ethanol and stained with Propidium Iodide (PI).
The histograms show the percentages of G0/G1 phase, S phase and G2/M phase of cell cycle analysis for
drug treated HeLa cells on Arthur™ (A) and competitor’s product F (B).

The cell cycle analysis of Arthur™ was highly consistent with the results obtained from competitor’s product F.