3D Spheroid

3D Spheroid Solution

Although two-dimensional (2D) cell cultures have long been the standard for drug development and tumor research, 3D spheroids or tumor cell aggregates more accurately resemble characteristics of tumors growing in vivo. 3D spheroid are an improved model for representing the intricate cell-cell and cell matrix interactions, and complex transport dynamics for nutrients and cells. 3D spheroids are superior to 2D cell cultures in testing gene expression for drug toxicology screening since they more closely resemble gene expression in vivo. 3D Spheroids have greater stability and a longer lifespan than 2D cell cultures, thus making them an improved predictor of in vivo drug efficacy and toxicity. “Spheroids are simple, widely used multicellular 3D models that form due to the tendency of adherent cells to aggregate. They can be generated from a broad range of cell types including tumor spheroids, embryonic bodies, hepatospheres, neurospheres, and mammospheres. 

3D multicellular spheroids can develop metabolic gradients that create heterogeneous cell populations with superior cell-to-cell and cell-to-ECM interactions.1 They offer a more physiologically relevant model as compared to 2D cell culture and can successfully mimic the microenvironment of a variety of tissue types in disease states. 3D Spheroid  3D Spheroid 

Spheroid models grown in Corning® spheroid micro plates can be used to create uniform embryonic bodies from induced pluripotent stem cells (iPSCs) that can be subsequently generated into high-purity neural stem cells (NSC) for the study of potential neural disease treatments.

3D tumor spheroid models generated with Corning spheroid micro plates closely mimic the in vivo tumor microenvironment. These spheroids – grown as either single cultures or more complex co-cultures with other cell types in the tumor microenvironment – offer an opportunity to better predict the therapeutic efficacy of cancer drug models in oncology drug discovery applications” (NIH).

Chinchilla Life Sciences Solutions

3D Spheroid