JuLI™ FL – Fluorescence Cell History Recorder

//JuLI™ FL – Fluorescence Cell History Recorder

JuLI™ FL – Fluorescence Cell History Recorder

$9,395.00

Numerous cells are widely used and being cultured to test the efficacy and toxicity of drugs in cancer therapies and regenerative medicines in combination with drug delivery, tissue engineering, and theragnostic investigations. In particular, analyzing cell behaviors and morphological changes from drug administration is critical for the comprehensive understanding of their mechanisms. The JuLI FL , is a smart Fluorescence-cell movie recorder and analyzer, designed for a variety of biological experiments.

JuLI FL  enables live-cell time-lapse images to be directly captured from within a cell culture incubator using culture dishes of various sizes.  The compact design of the system (scope unit) allows it to be installed in virtually any size incubator and prevents contamination by maintaining a sterile environment.  Operated remotely, the system’s control and display (station unit) allows convenient monitoring of cellular activity and time lapse imaging.

SKU: JULI-FLG04 Category:

Description

(GFP, Single set, 1 Scope & 1 Station)

A smart Fluorescence cell movie recorder and analyzer that works inside your incubator   JuLI FL  uses states-of-the-art optics to get live-cell images from various cell culture dishes and can perform cell-based assays, including cell counting, cell viability, and quantitative analysis of GFP/RFP expression.  It is able to detect the quantified cell confluence results with low variation and make a growth curve using image based analysis.

JuLI FL  is able to capture sequential time-lapse images which can be converted to movie files (.avi) automatically.  The compact design allows you to install the system in your cell-culture incubator easily.  It can be used to compare the control and experimental samples using a dual scope system (*optional) concurrently.

Features of the JuLI FL, Fluorescence live-cell movie recorder and analyzer

Versatility

  • Time-lapse image capturing & making a movie
  • Cell counting and viability
  • GFP or RFP expression level checking
  • Dual system available for comparative cell analysis (*optional)

Accuracy

  • Automated confluence detection & growth curve
  • Semi auto-focusing with low variations

 Ease of use

  • Intuitive graphic user interface & LCD touch screen
  • Compact size optimized for an cell culture incubator
  • Simple steps for the system setups and the operations
  • No calibration & maintenance required

Automated cell confluence detection – Quantified cell confluence results with low variation

10.1” color LCD touch screen – user friendly interface

Dual system (*optional) – Compare control and experimental samples using a dual system concurrently

Live cell image capture – U-2 OS Cells were transfected using the Neon™ transfection system (Life technologies) and 0.25µg of EGFP-N1 plasmid & RFP plasmid. 48 hours after transfection, the images are captured.

Time-lapse image capture and movie making – Cell growth images we captured for 63 hours with 20 minute intervals in U-2 OS GFP & RFP stable cell line.

Real time cell growth curves – HeLa cells growth were observed for 40 hours with 10 minute intervals and monolayer confluence analyzed for apoptosis assays, experimental group (channel 2) was treated with Sataurosporine.

Cell migration (wound healing) assay – U-2 OS stable cells were incubated for 100 hours after scratch. JuLI FL calculated the confluence with growth of surface unframed automatically.

[Wound healing progress graph] Wound confluence can be graphed to quantitatively analyze the recovering surface of the wound. (Bright field only)

Fluorescence expression level checking – JuLI FL counting software provides actual cell counts, viability, and automatically calculates cell concentration. It can also identify and measure the level of fluorescence (GFP or RFP expression).

Highly qualified technical support by NanoEnTek technical application specialists (HQ) and Chinchilla Life technicians.

  • Qualified specialists are ready to support you. From free training on basic operation to experimental support, we can help.

Video

Specifications

Items Specifications
Magnification CMOS
Camera 1280 x 960 pixels (1.3M)
Image resolution 1280 x 960 pixels (1.3M)
Export formats JPEG, TIFF, BMP, PNG (image), AVI (movie),
CSV (raw data)
Display 10.1” LCD touch screen
Light Source GFP channel (JULI-FLG04): Blue LED
RFP channel (JULI-FLR04): Green LED
Optical Filter GFP channel (JULI-FLG04): Excitation 466/40 nm, Emission 525/50 nm
RFP channel (JULI-FLR04): Excitation 525/50 nm, Emission 580LP
Counting time < 10 sec/test
Cell measurement range 1 x 10– 1 x 107 cell/µL
Optimal measurement range 1 x 10– 1 x 106 cell/µL
Cell size range 5 – 60 µm
Dimension & Weight Scope unit : 300 x 190 x 188 mm, 4.5 kg
Station unit : 282 x 285 x 160 mm, 3.2 kg
Storage 320 GB Hard drive, 4 GB USB drive
Power AC 100 – 240V, 1.9A, 100W, 50-60Hz
Operating Environment 5 – 40 °C, 20 – 95 %

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