Numerous cells are widely used and being cultured to test the efficacy and toxicity of drugs in cancer therapies and regenerative medicines in combination with drug delivery, tissue engineering, and theragnostic investigations. In particular, analyzing cell behaviors and morphological changes from drug administration is critical for the comprehensive understanding of their mechanisms. The JuLI FL , is a smart Fluorescence-cell movie recorder and analyzer, designed for a variety of biological experiments.
JuLI FL enables live-cell time-lapse images to be directly captured from within a cell culture incubator using culture dishes of various sizes. The compact design of the system (scope unit) allows it to be installed in virtually any size incubator and prevents contamination by maintaining a sterile environment. Operated remotely, the system’s control and display (station unit) allows convenient monitoring of cellular activity and time lapse imaging.
A smart Fluorescence cell movie recorder and analyzer that works inside your incubator JuLI FL uses states-of-the-art optics to get live-cell images from various cell culture dishes and can perform cell-based assays, including cell counting, cell viability, and quantitative analysis of GFP/RFP expression. It is able to detect the quantified cell confluence results with low variation and make a growth curve using image based analysis.
JuLI FL is able to capture sequential time-lapse images which can be converted to movie files (.avi) automatically. The compact design allows you to install the system in your cell-culture incubator easily. It can be used to compare the control and experimental samples using a dual scope system (*optional) concurrently.
Features of the JuLI FL, Fluorescence live-cell movie recorder and analyzer
- Time-lapse image capturing & making a movie
- Cell counting and viability
- GFP or RFP expression level checking
- Dual system available for comparative cell analysis (*optional)
- Automated confluence detection & growth curve
- Semi auto-focusing with low variations
Ease of use
- Intuitive graphic user interface & LCD touch screen
- Compact size optimized for an cell culture incubator
- Simple steps for the system setups and the operations
- No calibration & maintenance required
Automated cell confluence detection – Quantified cell confluence results with low variation
10.1” color LCD touch screen – user friendly interface
Dual system (*optional) – Compare control and experimental samples using a dual system concurrently
Live cell image capture – U-2 OS Cells were transfected using the Neon™ transfection system (Life technologies) and 0.25µg of EGFP-N1 plasmid & RFP plasmid. 48 hours after transfection, the images are captured.
Time-lapse image capture and movie making – Cell growth images we captured for 63 hours with 20 minute intervals in U-2 OS GFP & RFP stable cell line.
Real time cell growth curves – HeLa cells growth were observed for 40 hours with 10 minute intervals and monolayer confluence analyzed for apoptosis assays, experimental group (channel 2) was treated with Sataurosporine.
Cell migration (wound healing) assay – U-2 OS stable cells were incubated for 100 hours after scratch. JuLI FL calculated the confluence with growth of surface unframed automatically.
[Wound healing progress graph] Wound confluence can be graphed to quantitatively analyze the recovering surface of the wound. (Bright field only)
Fluorescence expression level checking – JuLI FL counting software provides actual cell counts, viability, and automatically calculates cell concentration. It can also identify and measure the level of fluorescence (GFP or RFP expression).
Highly qualified technical support by NanoEnTek technical application specialists (HQ) and Chinchilla Life technicians.
- Qualified specialists are ready to support you. From free training on basic operation to experimental support, we can help.